|
KMID : 1005220130280040373
|
|
Journal of Embryo Transfer
2013 Volume.28 No. 4 p.373 ~ p.379
|
|
|
In Vitro Culture of Primary Testicular Stromal Cells derived from Mouse with Different Genetic Background : Optimization of Culture Temperature
|
|
Park Hye-Jin
Yun Jung-Im
Choi Jung-Hoon
Lee Eun-Song
Gong Seung-Pyo
Lee Seung-Tae
|
|
|
Abstract
|
|
|
|
Spermatogonial stem cells (SSCs) developed into sperms through spermatogenesis have been utilized as a useful tool in the field of regenerative medicine and infertility. However, a small number of highly qualified SSCs are resided in the seminiferous tubule of testis, resulted in developing effective in-vitro culture system of SSCs for solving simultaneously quantitative and qualitative problems. Presently, SSCs can be enriched on testicular stromal cells (TSCs), but there are no systematic researches about TSC culture. Therefore, we tried to optimize culture condition of TSCs derived from mouse with different strains. For these, proliferation and viability were measured and compared by culturing ICR outbred or DBA/2 inbred mouse-derived TSCs at 35 or 37℃. In case of ICR strain, primary TSCs cultured at 37℃ showed significantly higher proliferation and viability than those at 35℃ and significant increase of proliferation and viability in sub-passaged TSCs was detected in the 35℃ culture condition. Moreover, sub-passage of primary TSCs at 35℃ induced no significant effects on proliferation and viability. In contrast, in case of DBA/2 strain, significantly improved proliferation were detected in the primary TSCs cultured at 35℃, which showed no significant difference in the viability, compared to those at 37℃. Furthermore, sub-passaged TSCs cultured at 37℃ showed no significant differences in proliferation and viability, compared to those at 35℃. However, with significant decrease of proliferation induced by sub-passage of primary TSCs at 35℃, no significant effects on proliferation and viability were resulted from sub-passage of primary TSCs at 37℃. From these results, culture temperature of primary TSCs derived from outbred and inbred strain of mouse could be separately optimized in primary culture and subculture.
|
|
|
KEYWORD
|
|
|
mouse , testicular stromal cell , culture temperature , proliferation , viability
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|